D inside the post. Acknowledgments: The authors extend their appreciation to Princess Nourah bint Abdulrahman University Researchers Supporting Project quantity (PNURSP2022R141), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. Conflicts of Interest: The authors declare no conflict of interest.Pharmaceuticals 2022, 15,27 ofAbbreviations AHF: acute heart failure, ALP: alkaline phosphatase, ALT: alanine aminotransferase, AST: aspartate aminotransferase, CV: central vein, DNM1L: dynamin-1-like protein, KC: Kupffer cell, MAPK: mitogen-activated protein kinase, MID51: mitochondrial dynamics protein, miRNA: microRNA, Mtf: Mitofusin, PCNA: proliferating cell nuclear antigen, PGC-1: peroxisome proliferator-activated receptor co-activator 1, rER: rough endoplasmic reticulum, ROS: reactive oxygen species, SOD: superoxide dismutase, STAT3: signal transducer and activator of transcription 3, and TEM: transmission electron microscopic.
Original ArticleKetogenic eating plan administration to mice following a high-fat-diet regimen promotes fat reduction, glycemic normalization and induces adaptations of ketogenic pathways in liver and kidneySouad Nasser 1, 4, Thomas Sol2, 4, Nathalie Vega 1, Thierry Thomas 2, Aneta Balcerczyk 3, Maura Strigini 2, 5, Luciano Pirola 1, , 5 ABSTRACT Objective: The ketogenic diet (KD), characterized by pretty limited dietary carbohydrate intake and employed as nutritional treatment for GLUT1deficiency syndromes and pharmacologically refractory epilepsy, might promote weight-loss and increase metabolic fitness, potentially alleviating the symptoms of osteoarthritis. Here, we have studied the effects of administration of a ketogenic diet regime in mice previously rendered obese by feeding a high fat diet plan (HFD) and submitted to surgical destabilization with the medial meniscus to mimic osteoarthritis. Solutions: 6-weeks old mice were fed an HFD for 10 weeks and then switched to a chow diet plan (CD), KD or maintained on a HFD for 8 weeks. Glycemia, b-hydroxybutyrate (BHB), body weight and fat mass had been compared amongst groups.Sulfamethoxazole-d4 In Vitro In liver and kidney, protein expression and histone post-translational modifications had been assessed by Western blot, and gene expression by quantitative Real-Time PCR.Gliotoxin site Final results: Just after a 10 weeks HDF feeding, administration for 8 weeks of a KD or CD induced a comparable weight reduction and decrease in fat mass, with much better glycemic normalization within the KD group.PMID:23912708 Histone b-hydroxybutyrylation, but not histone acetylation, was enhanced in the liver and kidney of mice fed the KD as well as the rate-limiting ketogenic enzyme HMGCS2 was upregulated e at the gene and protein level e in liver and, to an even higher extent, in kidney. KD-induced HMGCS2 overexpression may very well be dependent on FGF21, whose gene expression was increased by KD in liver. Conclusions: Over a period of eight weeks, KD is more efficient than a chow diet program to induce metabolic normalization. In addition to acting as a fuel molecule, BHB may exert its metabolic effects by way of modulation of the epigenome – by way of histone b-hydroxybutyrylation – and substantial transcriptional modulation in liver and kidney.2022 The Authors. Published by Elsevier GmbH. This is an open access short article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Keyword phrases Ketogenesis; Ketogenic eating plan; b-hydroxybutyrate; Histone PTMs; HMGCS2 1. INTRODUCTION Ketogenic diets, consisting of your practically full elimination of dietary carbohydrates, induce the body to depend on ketone bodies as main power sourc.
