Rabbit anti-Glucagon Polyclonal Antibody

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Product Details FAQ Citations(0) Video Pictures Documents |Overview |Description Rabbit polyclonal antibody to Glucagon |Synonym Glucagon |Host Rabbit |Specificity Recognizes endogenous levels of Glucagon protein. |Species Reactivity Human, Mouse, Rat, Bovine, Dog, Pig, Sheep |Applications WB 1:500 – 1:1000 , IHC 1:50 – 1:200 |Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Glucagon. The exact sequence is proprietary. |Properties |Purification The antibody was purified by immunogen affinity chromatography. |Clonality Polyclonal Antibody |Storage Temp. Shipped at 4 ° C Upon delivery aliquot and store at -20 ° C for one yearAvoid freeze/that cycles |Target |UniProt P01275 | Western blot analysis of Glucagon expression in LO2 (A), mouse kidney (B), H9C2 (C) whole cell lysates.| Immunohistochemical analysis of Glucagon staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Immunofluorescent analysis of Glucagon staining in H9C2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |Tips:This product is for research use only. Not for use in diagnostic prodcedures.